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实验室 常规仪器试剂耗材

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SL100688
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产品: 浏览次数:1542SL100688 
品牌: SignaGen
型号: SL100688
规格: 1.0ml
单价: 1280.00元/支
最小起订量: 1 支
供货总量: 10 支
发货期限: 自买家付款之日起 3 天内发货
有效期至: 长期有效
最后更新: 2020-08-13 11:45
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详细信息
SignaGen  高效转染试剂PolyJet™ DNA In Vitro Tranfection Reagent 
产地: SignaGen
货号: SL100688
规格: 0.1ml/0.5 ml/1.0ml
名称: PolyJet DNA In Vitro Transfection Reagent
产地
货号
产品名称
规格
价格()
SignaGen
SL100688
PolyJet DNA In Vitro Transfection Reagent (Trial Pack)
0.1 ml
230
SignaGen
SL100688
PolyJet DNA In Vitro Transfection Reagent
0.5 ml
680
SignaGen
SL100688
PolyJet DNA In Vitro Transfection Reagent
1.0 ml
1280


可生物降解的聚合物技术,是SignaGen的一项最新技术。在此技术的基础之上,SignaGen合成了新一代的可生物降解多价阳离子聚合物,以此聚合物作为载体,并成功研发了PolyJet DNA 体外转染试剂。PolyJet™转染试剂较市场上畅销的其他试剂,具有更高的转染效率及更低的毒性。PolyJet™转染试剂不但适用于HEK293, COS-7, NIH-3T3, HeLa, CHO等常用细胞系,同样适用于众多难转染的细胞。PolyJet™转染试剂与DNA室温孵育五分钟即可形成PolyJet/DNA 复合物,该复合物通过内吞作用被细胞吸收后,阳离子聚合物可以迅速、完全被降解,从而确保极低的细胞毒性(Figure1)。        
Figure 1. A Cartoon Showing Biodegradation of PolyJet DNA Transfection Reagent After Endocytosis of Transfection Complex
PolyJet™特点
²        载体的可降解,保证了更低的细胞毒性
²        对于>89 kbDNA同样具有卓越的转染效率
²        不仅适用于单个DNA的转染同样适用于共转染
²        可以获得高水平的蛋白表达
²        操作简单、快速
²        价格低于主流畅销产品的30%-50%
 PolyJet™广泛适用于哺乳动物细胞
窗体顶端
Cell Lines
窗体底端
Efficiency (% GFP)
Cell Lines
Efficiency (% GFP)
McArdle 7777
65-70%
SAOS-2
58%
Hep3D
67-76%
SN56
81%
SHEP
68-71%
MC3T3-E1
80%
3T3-442A
35%
Primary melanocyte
35%
COS-7
85-90%
K562
38%
CV-1
60%
L929
59%
D 407
70%
MCF-7
68%
DHD Pro.b
70%
MDCK
68%
3LL
80%
Neuro2A
86%
B16-F10
85%
NIH 3T3
76%
BAEC
51%
PC12
30%
BHK-21
80%
SH-SY5Y
25%
Ca Ski
88%
SiHa
60%
CaCo2
60%
SKOV3
65%
CHO
88%
Huh-7
70%
HCS-2/8
61%
IGROV1
35%
HEK-293
86%
DF-1, Chicken Embryonic Cell
50%
HeLa
88%
6CSFMEo
71%
HLMEC
72%
WEHI 231
26%
H-MVEC
59%
A549
75%
Huh-7D12
72%
LNCap
75
ATT20
46%
Prim. mouse keratinocyte
29%
SK-N-SH
29%
Prim. human skin fibroblast
50%
C2C12
46%
Prim. human pre-adipocyte
32%
PolyJet™同市售其他转染试剂相比具有更高的转染效率
Description
Based on our proprietary polymer synthesis technology, PolyJet™ DNA In Vitro Transfection Reagent is formulated as a biodegradable polymer based DNA transfection reagent that ensures effective and reproducible transfection on HEK293, COS-7, NIH-3T3, HeLa, CHO and a broad ranges of hard-to-transfect mammalian cells. PolyJet™ reagent is able to immobilize DNA migration during electrophoresis at very low concentration and form transfection complex within 5 minutes at RT. A remarkable feature of the reagent is the rapid and complete degradation of polymer after transfection complex endocytosis (Figure 1), leading to much less cytotoxicity. PolyJet™ reagent, 1.0 ml, is sufficient for 600 to 1200 transfections in 24 well plates or 300 to 600 transfections in 6 well plates, providing a very affordable alternative to the leading products for transfecting a variety of commonly used and hard-to-transfect mammalian cells.

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